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anti pp2aα  (Proteintech)


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    Structured Review

    Proteintech anti pp2aα
    Anti Pp2aα, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 55 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+pp2a%CE%B1/PPP2CA+Antibody/10__31083_slash_jin46980-117-58-64
    Average 94 stars, based on 55 article reviews
    anti pp2aα - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    SDS Page:

    Article Title: Acupuncture Modulates NMDAR-PP1/Calpain1-KCC2 Pathway to Ameliorate Spinal Hyperexcitability and Spastic Hemiplegia Induced by Ischemic Stroke
    Article Snippet: Proteins were extracted from the lumbar spinal ventral horn and concentrations were determined with a BCA kit (cat. no. AR0146; Boster; Wuhan, Hubei, China). .. After SDS-PAGE (8 and 10%) and PVDF membrane transfer (cat. no. IPVH00010; Millipore; Burlington, MA, USA), membranes were blocked and incubated with the following primary antibodies: anti-NMDAR1 (1:6000, cat. no. ab109182; Abcam), anti-KCC2 (1:10,000, cat. no. ab259969; Abcam), anti-phospho-KCC2 (Ser940) (1:3000, cat. no. CAY-29291-100; Cayman Chemical; Ann Arbor, MI, USA), anti-PP1Cγ (1:2000, cat. no. bs-5422R; Bioss; Beijing, China), anti-PP2Aα (1:2000, cat. no. 13482-1- AP; Proteintech), anti-Calpain1 (1:2000; WL03987; Wanleibio; Shenyang, Liaoning, China), anti-SPTAN1 (1:2000, cat. no. 31676-1-AP; Proteintech), and anti-β-actin (1:10,000, cat. no. 66009-1-Ig; Proteintech). .. Signals were imaged with the CFX Connect Real-Time System (BioRad; Hercules, CA, USA).

    Membrane:

    Article Title: Acupuncture Modulates NMDAR-PP1/Calpain1-KCC2 Pathway to Ameliorate Spinal Hyperexcitability and Spastic Hemiplegia Induced by Ischemic Stroke
    Article Snippet: Proteins were extracted from the lumbar spinal ventral horn and concentrations were determined with a BCA kit (cat. no. AR0146; Boster; Wuhan, Hubei, China). .. After SDS-PAGE (8 and 10%) and PVDF membrane transfer (cat. no. IPVH00010; Millipore; Burlington, MA, USA), membranes were blocked and incubated with the following primary antibodies: anti-NMDAR1 (1:6000, cat. no. ab109182; Abcam), anti-KCC2 (1:10,000, cat. no. ab259969; Abcam), anti-phospho-KCC2 (Ser940) (1:3000, cat. no. CAY-29291-100; Cayman Chemical; Ann Arbor, MI, USA), anti-PP1Cγ (1:2000, cat. no. bs-5422R; Bioss; Beijing, China), anti-PP2Aα (1:2000, cat. no. 13482-1- AP; Proteintech), anti-Calpain1 (1:2000; WL03987; Wanleibio; Shenyang, Liaoning, China), anti-SPTAN1 (1:2000, cat. no. 31676-1-AP; Proteintech), and anti-β-actin (1:10,000, cat. no. 66009-1-Ig; Proteintech). .. Signals were imaged with the CFX Connect Real-Time System (BioRad; Hercules, CA, USA).

    Incubation:

    Article Title: Acupuncture Modulates NMDAR-PP1/Calpain1-KCC2 Pathway to Ameliorate Spinal Hyperexcitability and Spastic Hemiplegia Induced by Ischemic Stroke
    Article Snippet: Proteins were extracted from the lumbar spinal ventral horn and concentrations were determined with a BCA kit (cat. no. AR0146; Boster; Wuhan, Hubei, China). .. After SDS-PAGE (8 and 10%) and PVDF membrane transfer (cat. no. IPVH00010; Millipore; Burlington, MA, USA), membranes were blocked and incubated with the following primary antibodies: anti-NMDAR1 (1:6000, cat. no. ab109182; Abcam), anti-KCC2 (1:10,000, cat. no. ab259969; Abcam), anti-phospho-KCC2 (Ser940) (1:3000, cat. no. CAY-29291-100; Cayman Chemical; Ann Arbor, MI, USA), anti-PP1Cγ (1:2000, cat. no. bs-5422R; Bioss; Beijing, China), anti-PP2Aα (1:2000, cat. no. 13482-1- AP; Proteintech), anti-Calpain1 (1:2000; WL03987; Wanleibio; Shenyang, Liaoning, China), anti-SPTAN1 (1:2000, cat. no. 31676-1-AP; Proteintech), and anti-β-actin (1:10,000, cat. no. 66009-1-Ig; Proteintech). .. Signals were imaged with the CFX Connect Real-Time System (BioRad; Hercules, CA, USA).



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